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anti ptch1 antibody  (Novus Biologicals)


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    Novus Biologicals anti ptch1 antibody
    Anti Ptch1 Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nb200+118/Patched+1%2FPTCH+Antibody+-+BSA+Free/pm36220081-705-119-127
    Average 90 stars, based on 7 article reviews
    anti ptch1 antibody - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Participation of the Immune System and Hedgehog Signaling in Neoangiogenesis Under Laser Photobiomodulation
    Article Snippet: All the antibodies used in the study were standardized (Anti-CD31/1:1000, SC-1506, Santa Cruz Biotech; Anti Ptch1/1:1000, NB200-118, Novus Biological; Anti-Gli-2/1:1000, GWB-CE7858, GenWay Biotech; Anti-Ihh/1:250, AB 39634, Abcam; Anti-NG2/1:200, MAB 5384, Millipore; Anti-Actina/1:200, M0851, DakoCytomation; Anti-CD8/1:10, AR0801, Biosource; Anti-CD68/1:200, MCA341R, AbD Serotec).

    Article Title: GANT61 Reduces Hedgehog Molecule (GLI1) Expression and Promotes Apoptosis in Metastatic Oral Squamous Cell Carcinoma Cells
    Article Snippet: The following primary antibodies were diluted in 1x PBS + 1% BSA: mouse polyclonal against Sonic Hedgehog (1:500, Novus Biologicals, Clone 5 H4, Cat. number NBP2-22126, Novus Biologicals, Centennial, CO, USA), rabbit polyclonal against Gli1 (1:500, Novus Biologicals, Cat. number NB600-600, Novus Biologicals, Centennial, CO, USA), rabbit polyclonal against Patched 1 (1:500, Novus Biologicals, Cat. number NB200-118, Novus Biologicals, Centennial, CO, USA), and rabbit polyclonal against Smoothened (1:1000, Abcam, Cat. number AB72130, Abcam, Cambridge, MA, USA).

    Immunohistochemistry:

    Article Title: Quiescent adult stem cells in murine teeth are regulated by Shh signaling.
    Article Snippet: The mechanisms regulating the maintenance of quiescent adult stem cells in teeth remain to be fully elucidated.. Our aim is to clarify the relationship between BrdU label-retaining cells (LRCs) and sonic hedgehog (Shh) signaling in murine teeth.. After prenatal BrdU labeling, mouse pups were analyzed during postnatal day 1 (P1) to week 5 (P5W).



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    Regulation of AKT and HH pathways in SCC-4 by stimulation with IGF-1 or fibroblast-conditioned media. SCC-4 cells were stimulated for 24 h with 10% FBS, 100 ng/mL rIGF-1 or 50% conditioned medium from control fibroblasts (MF1) or cells overexpressing IGF-1 (MF1-IGF-1). Western blot analysis was performed to detect ( A ) total AKT and phospho-Akt (S473), with band intensity measured and plotted using ImageJ; ( B ) GLI1, IHH, <t>PTCH1</t> and SMO band intensities were measured and plotted using ImageJ; ( C ) GLI1 mRNA expression was evaluated by RT-qPCR 6 h after stimulating cells with rIGF-1 or conditioned media. Data are presented as means ± SDs, bars represent comparisons between respective groups and (*) denotes statistical significance after applying the one-way ANOVA and Dunnett’s post-test, p < 0.05. CM: conditioned medium. ( D ) IHH immunostaining in MF1 and MF1-IGF-1 fibroblasts. The presence of the IHH ligand is shown in red, while nuclei were stained with DAPI (blue). Bars = 50 μm.
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    Regulation of AKT and HH pathways in SCC-4 by stimulation with IGF-1 or fibroblast-conditioned media. SCC-4 cells were stimulated for 24 h with 10% FBS, 100 ng/mL rIGF-1 or 50% conditioned medium from control fibroblasts (MF1) or cells overexpressing IGF-1 (MF1-IGF-1). Western blot analysis was performed to detect ( A ) total AKT and phospho-Akt (S473), with band intensity measured and plotted using ImageJ; ( B ) GLI1, IHH, <t>PTCH1</t> and SMO band intensities were measured and plotted using ImageJ; ( C ) GLI1 mRNA expression was evaluated by RT-qPCR 6 h after stimulating cells with rIGF-1 or conditioned media. Data are presented as means ± SDs, bars represent comparisons between respective groups and (*) denotes statistical significance after applying the one-way ANOVA and Dunnett’s post-test, p < 0.05. CM: conditioned medium. ( D ) IHH immunostaining in MF1 and MF1-IGF-1 fibroblasts. The presence of the IHH ligand is shown in red, while nuclei were stained with DAPI (blue). Bars = 50 μm.
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    Image Search Results


    Regulation of AKT and HH pathways in SCC-4 by stimulation with IGF-1 or fibroblast-conditioned media. SCC-4 cells were stimulated for 24 h with 10% FBS, 100 ng/mL rIGF-1 or 50% conditioned medium from control fibroblasts (MF1) or cells overexpressing IGF-1 (MF1-IGF-1). Western blot analysis was performed to detect ( A ) total AKT and phospho-Akt (S473), with band intensity measured and plotted using ImageJ; ( B ) GLI1, IHH, PTCH1 and SMO band intensities were measured and plotted using ImageJ; ( C ) GLI1 mRNA expression was evaluated by RT-qPCR 6 h after stimulating cells with rIGF-1 or conditioned media. Data are presented as means ± SDs, bars represent comparisons between respective groups and (*) denotes statistical significance after applying the one-way ANOVA and Dunnett’s post-test, p < 0.05. CM: conditioned medium. ( D ) IHH immunostaining in MF1 and MF1-IGF-1 fibroblasts. The presence of the IHH ligand is shown in red, while nuclei were stained with DAPI (blue). Bars = 50 μm.

    Journal: International Journal of Molecular Sciences

    Article Title: Effects of IGF-1 on Proliferation, Angiogenesis, Tumor Stem Cell Populations and Activation of AKT and Hedgehog Pathways in Oral Squamous Cell Carcinoma

    doi: 10.3390/ijms21186487

    Figure Lengend Snippet: Regulation of AKT and HH pathways in SCC-4 by stimulation with IGF-1 or fibroblast-conditioned media. SCC-4 cells were stimulated for 24 h with 10% FBS, 100 ng/mL rIGF-1 or 50% conditioned medium from control fibroblasts (MF1) or cells overexpressing IGF-1 (MF1-IGF-1). Western blot analysis was performed to detect ( A ) total AKT and phospho-Akt (S473), with band intensity measured and plotted using ImageJ; ( B ) GLI1, IHH, PTCH1 and SMO band intensities were measured and plotted using ImageJ; ( C ) GLI1 mRNA expression was evaluated by RT-qPCR 6 h after stimulating cells with rIGF-1 or conditioned media. Data are presented as means ± SDs, bars represent comparisons between respective groups and (*) denotes statistical significance after applying the one-way ANOVA and Dunnett’s post-test, p < 0.05. CM: conditioned medium. ( D ) IHH immunostaining in MF1 and MF1-IGF-1 fibroblasts. The presence of the IHH ligand is shown in red, while nuclei were stained with DAPI (blue). Bars = 50 μm.

    Article Snippet: The following primary antibodies were used: GLI1 (1:500; Novus #NB800, Centennial, CO, USA), PTCH1 (1:500; Novus #NB200-118 Centennial, CO, USA), SHH (1:500; Novus #NBP2-22126, Centennial, CO, USA), IHH (1:500; Abcam #EP1192, Branford, CT, USA), pan-cytokeratin (1:500; MyBiosource IML-91, San Diego, CA, USA), Nanog (1:200; Santa Cruz SC1732, Dallas, TX, USA) and SOX2 (1:200; Millipore AB5731, Darmstadt, Alemanha).

    Techniques: Control, Western Blot, Expressing, Quantitative RT-PCR, Immunostaining, Staining